Efecto de dos medios de fertilización en el desarrollo in vitro de embriones bovinos criollos

José Goicochea Vargas, Wilson Rondón Jorge, Fidel Acosta Pachorro, Yusep Gómez Marín, Magaly Montalvo Martin, Max Salvatierra Alor, Jéssica Martel Falcón, Oscar Ballarte Zevallos, Julio Díaz Zegarra, Marcelo Ratto Fuster

Producción científica: Contribución a una revistaArtículorevisión exhaustiva

Resumen

The objective of this study was to evaluate the in vitro embryo production rate up to the blastocyst stage with two fertilization media: FERT-TALP and modified TCM-199, grown in BOEC medium, from oocytes collected by aspiration of follicular ovaries of creole cows obtained from the slaughterhouse. In total, 281 oocytes were collected, 179 for FERT-TALP medium and 144 for modified TCM-199. The oocytes were subjected to the process of maturation, fertilization and embryo culture in vitro. An oocyte cleavage rate of 25.8% was obtained in FERT-TALP and 2.4% in modified TCM-199 at 18 hours post-fertilization. Likewise, at 48h, 87.7% of total cleavage was obtained in FER-TALP and 40.5% in modified TCM-199, whose percentage of embryos with 4 to 8 cells was 64.5 and 26.2%, respectively. In the morula and blastocyst phase, a rate of 10.3 and 3.9% was achieved in FERT-TALP, respectively, while none wwere achieved with the modified TCM-199 medium.

Título traducido de la contribuciónEffect of two fertilization medium on the in vitro development of Creole bovine embryos
Idioma originalEspañol
Número de artículoe18709
PublicaciónRevista de Investigaciones Veterinarias del Peru
Volumen32
N.º5
DOI
EstadoPublicada - 27 oct. 2021

Palabras clave

  • Embryos
  • FERT-TALP
  • In vitro
  • Native cattle
  • TCM-199

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