TY - JOUR
T1 - Efecto de dos medios de fertilización en el desarrollo in vitro de embriones bovinos criollos
AU - Vargas, José Goicochea
AU - Jorge, Wilson Rondón
AU - Pachorro, Fidel Acosta
AU - Marín, Yusep Gómez
AU - Martin, Magaly Montalvo
AU - Alor, Max Salvatierra
AU - Falcón, Jéssica Martel
AU - Zevallos, Oscar Ballarte
AU - Zegarra, Julio Díaz
AU - Fuster, Marcelo Ratto
N1 - Publisher Copyright:
© Los autores. Este artículo es publicado por la Rev Inv Vet Perú de la Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos. Este es un artículo de acceso abierto, distribuido bajo los términos de la licencia Creative Commons Atribución 4.0 Internacional (CC BY 4.0) [https://creativecommons.org/licenses/by/4.0/deed.es] que permite el uso, distribución y reproducción en cualquier medio, siempre que la obra original sea debidamente citada de su fuente original
PY - 2021/10/27
Y1 - 2021/10/27
N2 - The objective of this study was to evaluate the in vitro embryo production rate up to the blastocyst stage with two fertilization media: FERT-TALP and modified TCM-199, grown in BOEC medium, from oocytes collected by aspiration of follicular ovaries of creole cows obtained from the slaughterhouse. In total, 281 oocytes were collected, 179 for FERT-TALP medium and 144 for modified TCM-199. The oocytes were subjected to the process of maturation, fertilization and embryo culture in vitro. An oocyte cleavage rate of 25.8% was obtained in FERT-TALP and 2.4% in modified TCM-199 at 18 hours post-fertilization. Likewise, at 48h, 87.7% of total cleavage was obtained in FER-TALP and 40.5% in modified TCM-199, whose percentage of embryos with 4 to 8 cells was 64.5 and 26.2%, respectively. In the morula and blastocyst phase, a rate of 10.3 and 3.9% was achieved in FERT-TALP, respectively, while none wwere achieved with the modified TCM-199 medium.
AB - The objective of this study was to evaluate the in vitro embryo production rate up to the blastocyst stage with two fertilization media: FERT-TALP and modified TCM-199, grown in BOEC medium, from oocytes collected by aspiration of follicular ovaries of creole cows obtained from the slaughterhouse. In total, 281 oocytes were collected, 179 for FERT-TALP medium and 144 for modified TCM-199. The oocytes were subjected to the process of maturation, fertilization and embryo culture in vitro. An oocyte cleavage rate of 25.8% was obtained in FERT-TALP and 2.4% in modified TCM-199 at 18 hours post-fertilization. Likewise, at 48h, 87.7% of total cleavage was obtained in FER-TALP and 40.5% in modified TCM-199, whose percentage of embryos with 4 to 8 cells was 64.5 and 26.2%, respectively. In the morula and blastocyst phase, a rate of 10.3 and 3.9% was achieved in FERT-TALP, respectively, while none wwere achieved with the modified TCM-199 medium.
KW - Embryos
KW - FERT-TALP
KW - In vitro
KW - Native cattle
KW - TCM-199
UR - http://www.scopus.com/inward/record.url?scp=85119021050&partnerID=8YFLogxK
U2 - 10.15381/rivep.v32i5.18709
DO - 10.15381/rivep.v32i5.18709
M3 - Artículo
AN - SCOPUS:85119021050
SN - 1682-3419
VL - 32
JO - Revista de Investigaciones Veterinarias del Peru
JF - Revista de Investigaciones Veterinarias del Peru
IS - 5
M1 - e18709
ER -